Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding (2024)

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Applied Sciences

Volume 14

Issue 12

10.3390/app14125243

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Article

by

Yinsong Liu

1,2,3,

Xiumei Zhang

1,2,

Xiaolin Wu

3,4,

Zhaowei Hou

3,5,

Min Wang

1,2 and

Erlong Yang

2,*

1

Sanya Offshore Oil & Gas Research Institute, Northeast Petroleum University, Sanya 572024, China

2

Key Laboratory of Enhanced Oil Recovery, Northeast Petroleum University, Ministry of Education, Daqing 163000, China

3

State Key Laboratory of Continental Shale Oil, Daqing 163000, China

4

Daqing Oilfield Co., Ltd., Daqing 163000, China

5

Exploration and Development Research Institute of Daqing Oilfield Co., Ltd., Daqing 163000, China

*

Author to whom correspondence should be addressed.

Appl. Sci. 2024, 14(12), 5243; https://doi.org/10.3390/app14125243

Submission received: 24 May 2024 / Revised: 9 June 2024 / Accepted: 10 June 2024 / Published: 17 June 2024

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Abstract

The microbial communities associated with alkaline–surfactant–polymer (ASP)-flooded reservoirs have rarely been investigated. In this study, high-throughput sequencing was used to analyse the indigenous microbial communities in two different blocks, the water flooding after the alkaline–surfactant–polymer flooding block and the alkaline–surfactant–polymer flooding block, and to ascertain the optimal stage for the implementation of indigenous microbial oil recovery technology. The different displacement blocks had significant effects on the indigenous microbial community at the genus level according to an alpha diversity analysis and community composition. In water flooding after alkaline–surfactant–polymer flooding, the dominant genus of Pseudomonas exceeded 30%, increasing to 52.1% in alkaline–surfactant–polymer flooding, but alpha diversity decreased. Through a co-occurrence network analysis, it was found that the complexity of the water flooding after alkaline–surfactant–polymer flooding was higher than that of alkaline–surfactant–polymer flooding. This means that the water flooding ecosystem after alkaline–surfactant–polymer flooding was more stable and less susceptible to external environmental influences. In addition, there were significant differences in the functional redundancy of microbial communities in different blocks. In summary, the optimal stage for implementing local microbial oil recovery technology may be water flooding after alkaline–surfactant–polymer flooding.

Keywords: indigenous microbial community structure; high-throughput sequencing; MEOR; alkaline–surfactant–polymer flooding

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MDPI and ACS Style

Liu, Y.; Zhang, X.; Wu, X.; Hou, Z.; Wang, M.; Yang, E. Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding. Appl. Sci. 2024, 14, 5243. https://doi.org/10.3390/app14125243

AMA Style

Liu Y, Zhang X, Wu X, Hou Z, Wang M, Yang E. Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding. Applied Sciences. 2024; 14(12):5243. https://doi.org/10.3390/app14125243

Chicago/Turabian Style

Liu, Yinsong, Xiumei Zhang, Xiaolin Wu, Zhaowei Hou, Min Wang, and Erlong Yang. 2024. "Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding" Applied Sciences 14, no. 12: 5243. https://doi.org/10.3390/app14125243

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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MDPI and ACS Style

Liu, Y.; Zhang, X.; Wu, X.; Hou, Z.; Wang, M.; Yang, E. Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding. Appl. Sci. 2024, 14, 5243. https://doi.org/10.3390/app14125243

AMA Style

Liu Y, Zhang X, Wu X, Hou Z, Wang M, Yang E. Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding. Applied Sciences. 2024; 14(12):5243. https://doi.org/10.3390/app14125243

Chicago/Turabian Style

Liu, Yinsong, Xiumei Zhang, Xiaolin Wu, Zhaowei Hou, Min Wang, and Erlong Yang. 2024. "Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding" Applied Sciences 14, no. 12: 5243. https://doi.org/10.3390/app14125243

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Research on Microbial Community Structure in Different Blocks of Alkaline–Surfactant–Polymer Flooding to Confirm Optimal Stage of Indigenous Microbial Flooding (11)

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